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Anti-FBXL5 Antibody
Catalog #: 144-05602
FBXL5 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UKA1 |
| Gene Id | 26234 |
| Gene Symbols | FBXL5 |
| Protein Name / Synonyms | FBXL5, FBL4, FBL5, FLR1, F-box/LRR-repeat protein 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
76kDa/78kDa
Observed MW
79kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human FBXL5 (NP_036293.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FBXL5 antibody (144-05602) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using FBXL5 antibody (144-05602) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using FBXL5 antibody (144-05602) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.