Anti-FDFT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P37268
Gene Id	2222
Gene Symbols	FDFT1
Protein Name / Synonyms	FDFT1, DGPT, ERG9, SQS, SS, squalene synthase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

35kDa/38kDa/40kDa/43kDa/48kDa

Observed MW

48kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human FDFT1 (NP_004453.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FDFT1 antibody (144-06229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - FDFT1 antibody (144-06229)

Immunohistochemistry of paraffin-embedded mouse testis using FDFT1 antibody (144-06229) at dilution of 1:100 (20x lens).
Immunohistochemistry - FDFT1 antibody (144-06229)

Immunohistochemistry of paraffin-embedded mouse brain using FDFT1 antibody (144-06229) at dilution of 1:100 (40x lens).
Immunohistochemistry - FDFT1 antibody (144-06229)

Immunohistochemistry of paraffin-embedded rat spinal cord using FDFT1 antibody (144-06229) at dilution of 1:100 (40x lens).
Immunohistochemistry - FDFT1 antibody (144-06229)