Anti-FEN1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P39748
Gene Id	2237
Gene Symbols	FEN1
Protein Name / Synonyms	FEN1
Clonality	Polyclonal
Recommended Applications	Western Blotting, IF, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Specificity

Other Names

Flap endonuclease 1, FEN-1, 31--, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1, FEN1, RAD2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42593

Recommended Dilutions

WB: 1:1000

Western blot analysis of lysates from Jurkat,Hela cell line (from left to right),using FEN1 Antibody(C10585). C10585 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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