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Anti-FEN1 Antibody
Catalog #: 144-01175
FEN1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P39748 |
| Gene Id | 2237 |
| Gene Symbols | FEN1 |
| Protein Name / Synonyms | FEN1, FEN-1, MF1, RAD2, flap endonuclease 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:10 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
35kDa/42kDa
Observed MW
Refer to Figures
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN1 (NP_004102.1).
Modification
Unmodified
Storage
-20°C
Images
Immunohistochemistry of paraffin-embedded human lung cancer using FEN1 antibody (144-01175) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using FEN1 antibody (144-01175) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using FEN1 antibody (144-01175).
Immunofluorescence analysis of HeLa cells using FEN1 antibody (144-01175).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FEN1 antibody (144-01175). Western blot was performed from the immunoprecipitate using FEN1 antibody (144-01175) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.