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Anti-FGF2 Antibody
Catalog #: 144-00235
FGF2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09038 |
| Gene Id | 2247 |
| Gene Symbols | FGF2 |
| Protein Name / Synonyms | FGF2, BFGF, FGF-2, FGFB, HBGF-2, fibroblast growth factor 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
17kDa/21kDa/22kDa/30kDa
Observed MW
20kDa, 36kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 143-288 of human FGF2 (NP_001997.5).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FGF2 antibody (144-00235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using FGF2 antibody (144-00235) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using FGF2 antibody (144-00235) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using FGF2 antibody (144-00235) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse liver using FGF2 antibody (144-00235) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using FGF2 antibody (144-00235) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FGF2 antibody (144-00235) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.