Anti-FGFR2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat, Non-Human Primate (rhesus monkey)
Host Species	Rabbit
Accession Number	P21802
Gene Id	2263
Gene Symbols	FGFR2
Protein Name / Synonyms	FGFR2 (N-term)
Clonality	Polyclonal
Preparation	This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human FGFR2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF, FC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.

Specificity

Other Names

FGFR2; BEK; KGFR; KSAM; Fibroblast growth factor receptor 2; K-sam; Keratinocyte growth factor receptor; CD_antigen=CD332; Flags: Precursor

NCBI Accession #

NP_000132.3;NP_001138385.1;NP_001138386.1;NP_001138387.1;NP_001138388.1;NP_001138389.1;NP_001138390.1;NP_001138391.1;NP_075259.4;NP_075418.1

Other Accession #

P21803

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92025

Recommended Dilutions

WB: 1:1000; IF: 1:50-100; FC: 1:10-50; IHC: 1:50-100

	FGFR2 Antibody (N-term) (Cat# 102-17497) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).
	FGFR2 Antibody (N-term) (Cat# 102-17497) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).
	Confocal immunofluorescent analysis of FGFR2 Antibody (N-term)(Cat# 102-17497) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	FGFR2 Antibody (N-term) (Cat# 102-17497) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).