Anti-FH Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P07954
Gene Id	2271
Gene Symbols	FH
Protein Name / Synonyms	FH, FMRD, HLRCC, LRCC, MCL, MCUL1, fumarate hydratase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW

50kDa/54kDa

Observed MW

50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human FH (NP_000134.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FH antibody (144-05688) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - FH Polyclonal Antibody (144-05688)

Immunofluorescence analysis of U2OS cells using FH antibody (144-05688). Blue: DAPI for nuclear staining.
Immunofluorescence - FH Polyclonal Antibody (144-05688)

Expiration:

12 months from the date of shipment when stored properly.

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