Anti-FHIT Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P49789
Gene Id	2272
Gene Symbols	FHIT
Protein Name / Synonyms	FHIT, AP3Aase, FRA3B, fragile histidine triad
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

16kDa

Observed MW

35kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FHIT antibody (144-01196) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - FHIT antibody (144-01196)

Immunohistochemistry of paraffin-embedded rat heart using FHIT antibody (144-01196) at dilution of 1:100 (40x lens).
Immunohistochemistry - FHIT antibody (144-01196)

Immunohistochemistry of paraffin-embedded mouse heart using FHIT antibody (144-01196) at dilution of 1:100 (40x lens).
Immunohistochemistry - FHIT antibody (144-01196)

Expiration:

12 months from the date of shipment when stored properly.

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