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Anti-FHL5 Antibody
Catalog #: 144-61184
FHL5 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q5TD97 |
| Gene Id | 9457 |
| Gene Symbols | FHL5 |
| Protein Name / Synonyms | FHL5, 1700027G07Rik, ACT, FHL-5, dJ393D12.2, four and a half LIM domains 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Polymorphisms in this gene may be associated with susceptibility for migraine headaches. Alternative splicing results in multiple transcript variants encoding the same protein.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
32kDa
Observed MW
35kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-284 of human FHL5 (NP_065228.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HepG2 cells, using FHL5 antibody (144-015756) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.