Anti-FKBP3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q00688
Gene Id	2287
Gene Symbols	FKBP3
Protein Name / Synonyms	FKBP3, FKBP-25, FKBP-3, FKBP25, PPIase, FK506 binding protein 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:100

Format

Calculated MW

25kDa

Observed MW

30kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human FKBP3 (NP_002004.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FKBP3 antibody (144-06907) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - FKBP3 Polyclonal Antibody (144-06907)

Immunofluorescence analysis of HeLa cells using FKBP3 antibody (144-06907). Blue: DAPI for nuclear staining.
Immunofluorescence - FKBP3 Polyclonal Antibody (144-06907)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug FKBP3 antibody (144-06907). Western blot was performed from the immunoprecipitate using FKBP3 antibody (144-06907) at a dilition of 1:1000.
Immunoprecipitation - FKBP3 Polyclonal Antibody (144-06907)

Expiration:

12 months from the date of shipment when stored properly.

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