Anti-FKBP8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q14318
Gene Id	23770
Gene Symbols	FKBP8
Protein Name / Synonyms	FKBP8, FKBP38, FKBPr38, FK506 binding protein 8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Unlike the other members of the family, this encoded protein does not seem to have PPIase/rotamase activity. It may have a role in neurons associated with memory function.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

27kDa/44kDa

Observed MW

55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human FKBP8 (NP_036313.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FKBP8 antibody (144-07085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - FKBP8 antibody (144-07085)

Immunofluorescence analysis of A549 cells using FKBP8 antibody (144-07085). Blue: DAPI for nuclear staining.
Immunofluorescence - FKBP8 antibody (144-07085)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FKBP8 antibody (144-07085). Western blot was performed from the immunoprecipitate using FKBP8 antibody (144-07085) at a dilition of 1:1000.
Immunoprecipitation - 1ug FKBP8 antibody (144-07085)

Expiration:

12 months from the date of shipment when stored properly.

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