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Anti-Flag Antibody
Catalog #: 130-10296
Mouse Anti-Human Flag Antibody. Clone: 1F5-G4-F9, 3C9-F2-B6, and 5E8-F1-D5.
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Clone | 1F5-G4-F9, 3C9-F2-B6, 5E8-F1-D5 |
| Preparation | Immunogen was polypeptidet of Flag. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:80, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion. RayBiotech's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with RayBiotech).
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.