Anti-FLI1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q01543
Gene Id	2313
Gene Symbols	FLI1
Protein Name / Synonyms	FLI1, EWSR2, SIC-1, BDPLT21, Fli-1 proto-oncogene, ETS transcription factor
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200

Format

Calculated MW

29kDa/43kDa/47kDa/50kDa

Observed MW

43kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FLI1 antibody (144-05644) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - FLI1 Polyclonal Antibody (144-05644)

Immunohistochemistry of paraffin-embedded human lung cancer using FLI1 antibody (144-05644) at dilution of 1:100 (40x lens).
Immunohistochemistry - FLI1 Polyclonal Antibody (144-05644)

Immunohistochemistry of paraffin-embedded human uterine cancer using FLI1 antibody (144-05644) at dilution of 1:100 (40x lens).
Immunohistochemistry - FLI1 Polyclonal Antibody (144-05644)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug FLI1 antibody (144-05644). Western blot was performed from the immunoprecipitate using FLI1 antibody (144-05644) at a dilition of 1:1000.
Immunoprecipitation - FLI1 Polyclonal Antibody (144-05644)

Expiration:

12 months from the date of shipment when stored properly.

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