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Anti-FLNB Antibody
Catalog #: 144-02481
FLNB Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75369 |
| Gene Id | 2317 |
| Gene Symbols | FLNB |
| Protein Name / Synonyms | FLNB, ABP-278, ABP-280, AOI, FH1, FLN-B, FLN1L, LRS1, SCT, TABP, TAP, filamin-B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:100
IHC 1:50 - 1:100
IF 1:20 - 1:100
Format
Calculated MW
230-281kDa
Observed MW
298kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FLNB antibody (144-02481) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using FLNB antibody (144-02481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (144-02481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using FLNB antibody (144-02481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (144-02481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using FLNB antibody (144-02481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using FLNB antibody (144-02481) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FLNB antibody (144-02481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HUVEC cells using FLNB antibody (144-02481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FLNB antibody (144-02481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using FLNB antibody (144-02481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.