3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-FN1 Antibody
Catalog #: 144-12932
FN1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P02751 |
| Gene Id | 2335 |
| Gene Symbols | FN1 |
| Protein Name / Synonyms | FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, fibronectin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 2.05 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
71-73kDa/221-272kDa
Observed MW
290kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human FN1 (NP_002017.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FN1 antibody (144-12932) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using FN1 antibody (144-12932) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FN1 antibody (144-12932) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using FN1 antibody (144-12932) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse leydig cells using FN1 antibody (144-12932) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.