Anti-FOS Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P01100
Gene Id	2353
Gene Symbols	FOS
Protein Name / Synonyms	FOS, AP-1, C-FOS, p55, proto-oncogene c-Fos
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

28kDa/36kDa/40kDa

Observed MW

70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 211-380 of human FOS (NP_005243.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FOS antibody (144-00236) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - FOS Polyclonal Antibody (144-00236)

Immunohistochemistry of paraffin-embedded human lung cancer using C-FOS antibody (144-00236) at dilution of 1:150 (40x lens).
Immunohistochemistry - FOS Polyclonal Antibody (144-00236)

Immunohistochemistry of paraffin-embedded mouse heart using C-FOS antibody (144-00236) at dilution of 1:150 (40x lens).
Immunohistochemistry - FOS Polyclonal Antibody (144-00236)

Expiration:

12 months from the date of shipment when stored properly.