Anti-FOXO3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	56484
Gene Symbols	FOXO3
Protein Name / Synonyms	FOXO3, AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, forkhead box O3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

71kDa

Observed MW

90kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FOXO3 (NP_062714.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FOXO3 antibody (144-00102) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - FOXO3 Polyclonal Antibody (144-00102)

Immunohistochemistry of paraffin-embedded rat brain using FOXO3 antibody (144-00102) at dilution of 1:100 (40x lens).
Immunohistochemistry - FOXO3 Polyclonal Antibody (144-00102)

Immunohistochemistry of paraffin-embedded human liver injury using FOXO3 antibody (144-00102) at dilution of 1:100 (40x lens).
Immunohistochemistry - FOXO3 Polyclonal Antibody (144-00102)

Immunofluorescence analysis of U2OS cells using FOXO3 antibody (144-00102) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - FOXO3 Polyclonal Antibody (144-00102)

Expiration:

12 months from the date of shipment when stored properly.

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