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Anti-FTH1 Antibody
Catalog #: 144-01144
FTH1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P02794 |
| Gene Id | 2495 |
| Gene Symbols | FTH1 |
| Protein Name / Synonyms | FTH1, FHC, FTH, FTHL6, HFE5, PIG15, PLIF, ferritin heavy chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Cardiovascular Disease, T Helper Cells, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
21kDa
Observed MW
20kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human FTH1 (NP_002023.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FTH1 antibody (144-01144) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug FTH1 antibody (144-01144). Western blot was performed from the immunoprecipitate using FTH1 antibody (144-01144) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.