Anti-FXN Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16595
Gene Id	2395
Gene Symbols	FXN
Protein Name / Synonyms	FXN, CyaY, FA, FARR, FRDA, X25, frataxin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

19kDa/21kDa/23kDa

Observed MW

13kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN (NP_000135.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FXN antibody (144-011785) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - FXN Polyclonal Antibody (144-011785)

Immunofluorescence analysis of 293T cells using FXN antibody (144-011785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - FXN Polyclonal Antibody (144-011785)

Immunofluorescence analysis of HeLa cells using FXN antibody (144-011785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - FXN Polyclonal Antibody (144-011785)

Expiration:

12 months from the date of shipment when stored properly.

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