RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-FXR1 Antibody

Catalog #: 144-05942

FXR1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P51114
Gene Id
8087
Gene Symbols
FXR1
Protein Name / Synonyms
FXR1, FXR1P, FMR1 autosomal homolog 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCancer, Inflammation
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
59kDa/60kDa/69kDa
Observed MW
70kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FXR1 (NP_005078.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FXR1 antibody (144-05942) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - FXR1 Polyclonal Antibody (144-05942)

Immunofluorescence analysis of U2OS cells using FXR1 antibody (144-05942) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - FXR1 Polyclonal Antibody (144-05942)

Expiration:
12 months from the date of shipment when stored properly.