Anti-FYN (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P06241
Gene Id	2534
Gene Symbols	FYN
Protein Name / Synonyms	FYN (N-term)
Clonality	Polyclonal
Preparation	This FYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of human FYN.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC, IHC-P
Research Area	Angiogenesis, Cardiovascular Disease, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein.

Specificity

Predicted Reactivity

Rat, Bovine, Chicken

Other Names

FYN; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; p59-Fyn

NCBI Accession #

NP_002028.1;NP_694592.1;NP_694593.1

Other Accession #

Q62844; P39688; Q05876; A0JNB0

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

60762

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:50-100

	Western blot analysis of FYN (arrow) using rabbit polyclonal FYN Antibody (N-term) (Cat# 102-17575). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.
	Western blot analysis of anti-FYN (N-term) Pab (Cat# 102-17575) in mouse liver tissue lysate. FYN (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	FYN Antibody (N-term) (Cat# 102-17575) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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