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Anti-GABARAP Antibody
Catalog #: 144-12568
GABARAP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95166 |
| Gene Id | 11337 |
| Gene Symbols | GABARAP |
| Protein Name / Synonyms | GABARAP, ATG8A, GABARAP-a, MM46, GABA type A receptor-associated protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Neuroscience, Autophagy, Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
13kDa
Observed MW
14kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABARAP (NP_009209.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GABARAP antibody (144-12568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using GABARAP antibody (144-12568) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using GABARAP antibody (144-12568) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using GABARAP antibody (144-12568) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using GABARAP antibody (144-12568) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP antibody (144-12568) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using GABARAP antibody (144-12568) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.