Anti-GABARAP (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O95166
Gene Id	11337
Gene Symbols	GABARAP
Protein Name / Synonyms	GABARAP (N-term)
Clonality	Polyclonal
Preparation	This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GABARAP.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, IHC-P, Western Blotting
Research Area	Neuroscience, Autophagy, Ras/Rap Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Rabbit

Other Names

GABARAP; FLC3B; Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; MM46

NCBI Accession #

NP_009209.1

Other Accession #

P60517; Q8MK68; Q9DCD6; Q9GJW7

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

13918

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:10-50

	Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-13733 GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
	Western blot analysis of GABARAP (arrow) using purified Pab (Cat# 102-13733). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).
	Rabbit anti-GABARAP RayBiotech 102-13733 1:1000 dilution in 5% scimmed milk powder. Secondary antibody goat anti-rabbit-HRP ECL detection 30 sec on X-ray film.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody (N-term)(Cat# 102-13733), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	GABARAP antibody was tested in mouse MEFs and mouse motor neurons. Cells were fixed in 3% PFA and permeabilized with 100% methanol. Antibodies used were RayBiotech anti-GABARAP (1:500, labeled with anti-rabbit 488 Alexa) and Covance anti-Tuj1 (1:1000, labeled with anti-mouse 647).