Anti-GAD2 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q05329
Gene Id	2572
Gene Symbols	GAD2
Protein Name / Synonyms	GAD2 (Center)
Clonality	Polyclonal
Preparation	This GAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human GAD2.
Concentration (lot specific)	0.470 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Neuroscience
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Specificity

Predicted Reactivity

Mouse, Rat, Pig

Other Names

GAD2; GAD65; Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase 65 kDa isoform

NCBI Accession #

NP_000809.1;NP_001127838.1

Other Accession #

Q05683; P48321; P48320; NP_001127838.1; NP_000809.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

65411

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	GAD2 Antibody (Center) (Cat# 102-10949) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow).
	GAD2 Antibody (Center) (Cat# 102-10949)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of GAD2 Antibody (Center)(Cat# 102-10949) with 293 cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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