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Anti-GAD65 Antibody
Catalog #: 107-11048
GAD65 antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 2572 |
| Protein Name / Synonyms | GAD65 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
GAD65 antibody, MGC161605 antibody, MGC161607 antibody, GAD2 antibody, 65 kDa glutamic acid decarboxylase antibody, glutamate decarboxylase 65 kDa isoform antibody, GAD-65 antibody, glutamate decarboxylase 2 antibody, Glutamate decarboxylase-2 (pancreas) antibody, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
65 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 353 and 585 of GAD65 (Uniprot ID# Q05329)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAD65(107-11048) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.
GAD65 antibody detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody (107-11048) dilution: 1:500.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody (107-11048) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
107-11048 diluted at 1:5000
A: mouse brain
7.5% SDS PAGE
107-11048 diluted at 1:5000
Sample (50 ug of whole cell lysate)
A: rat brain
7.5% SDS PAGE
107-11048 diluted at 1:1000
A: rat brain
7.5% SDS PAGE
107-11048 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.