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Anti-GAPDH (316-335aa) Antibody
Catalog #: 130-10919-1000
Product Description
Specifications
| Size | 1000 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04406 |
| Gene Id | 2597 |
| Gene Symbols | GAPDH GAPD CDABP0047 OK/SW-cl.12 |
| Protein Name / Synonyms | GAPDH (316-335aa) |
| Clonality | Polyclonal |
| Immunogen | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen GAPDH (316-335aa):NEFGYSNRVVDLMAHMASKEC. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Immunogen Sequence | NEFGYSNRVVDLMAHMASKEC |
| Recommended Applications | ELISA |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation.
Western Blot
130-10919 (pAb): 1:2000
8 % SDS-PAGE
Goat anti-rabbit IgG-HRP: 1:10000
Lane: M: protein marker 5 µ
- Hela cell cytoplasm fraction 10 µl
- 293T cell cytoplasm fraction 10 µl
- A549 cell cytoplasm fraction 10 µl
Simple Western
Simple Western analysis for anti-Human GAPDH antibody using different cell lysates
Indirect Fluorescent Antibody (IFA) Assay
130-10919 (1:1000)
Alexa Fluor 488 goat anti-rabbit (1:500)
Hela cells
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.