Anti-GAS2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O43903
Gene Id	2620
Gene Symbols	GAS2
Protein Name / Synonyms	GAS2, GAS-2, growth arrest specific 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

15kDa/34kDa

Observed MW

37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human GAS2 (NP_005247.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GAS2 antibody (144-01168) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - GAS2 antibody (144-01168)

Immunohistochemistry of paraffin-embedded human liver cancer using GAS2 antibody (144-01168) at dilution of 1:200 (40x lens).
Immunohistochemistry - GAS2 antibody (144-01168)

Immunofluorescence analysis of HeLa cells using GAS2 antibody (144-01168). Blue: DAPI for nuclear staining.
Immunofluorescence - GAS2 antibody (144-01168)

Expiration:

12 months from the date of shipment when stored properly.

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