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Anti-GATA2 Antibody
Catalog #: 144-00677
GATA2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P23769 |
| Gene Id | 2624 |
| Gene Symbols | GATA2 |
| Protein Name / Synonyms | GATA2, DCML, IMD21, MONOMAC, NFE1B, GATA binding protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
49kDa/50kDa
Observed MW
51kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GATA2 (NP_116027.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GATA2 antibody (144-00677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse brain using GATA2 antibody (144-00677) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GATA2 antibody (144-00677) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.