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Anti-GC Antibody
Catalog #: 144-05709
GC Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P02774 |
| Gene Id | 2638 |
| Gene Symbols | GC |
| Protein Name / Synonyms | GC, DBP, DBP/GC, GRD3, Gc-MAF, GcMAF, HEL-S-51, VDBG, VDBP, vitamin D-binding protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
39kDa/52kDa/55kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 324-493 of human GC (NP_001191236.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GC antibody (144-05709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 120s.
Immunofluorescence analysis of U2OS cells using GC antibody (144-05709). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.