GEMIN2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O14893 |
| Gene Id | 8487 |
| Gene Symbols | GEMIN2 |
| Protein Name / Synonyms | GEMIN2, SIP1, SIP1-delta, gem-associated protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
4kDa/28kDa/29kDa/31kDa
Observed MW
35kDa
Immunogen
A synthetic peptide of human GEMIN2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells, using GEMIN2 antibody (144-03082) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using GEMIN2 Antibody (144-03082) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using GEMIN2 Antibody (144-03082) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lymphonodus using GEMIN2 Antibody (144-03082) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GEMIN2 Antibody (144-03082) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using GEMIN2 Antibody (144-03082) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GEMIN2 Antibody (144-03082) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using GEMIN2 Antibody (144-03082) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using GEMIN2 Antibody (144-03082) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GEMIN2 Antibody (144-03082) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using GEMIN2 antibody (144-03082). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.