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Anti-GFAP Antibody
Catalog #: 130-10037
Rabbit Anti-Human GFAP Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P14136 |
| Gene Id | 2670 |
| Gene Symbols | GFAP |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from Human GFAP. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000(at least detecting 12.5ng/ml)) |
| Antigen Catalog Number | 230-00034 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Neuroscience, Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Glial fibrillary acidic protein, or GFAP, is an intermediate filament (IF) protein belonging to the type III subclass of IF proteins. Like other IF proteins, GFAP is composed of an amino terminal head domain, a central rod domain and a carboxy terminal tail domain. GFAP is specifically found in astroglia, a cell type which is highly responsive to neurologic insults. Astrogliosis is found to be a result of mechanical trauma, AIDS dementia, prion infection and inflammatory demylination diseases, and is accompanied by an increase in GFAP expression. GFAP is an immunohistochemical marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat Smooth Muscle has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month. Store at -20°C if over months. Minimize freezing and thawing when use.
Related Products
Rabbit Anti-Bovine GFAP(cat#DS-PB-00867) Goat Anti-Human GFAP (C-terminus)(cat#DS-PB-02261)
References
- Quinlan RA, Brenner M, Goldman JE, Messing A. GFAP and its role in Alexander disease. Exp Cell Res. 2007 Jun 10;313(10):2077-87.
- Zhu H, Dahlström A. Glial fibrillary acidic protein-expressing cells in the neurogenic regions in normal and injured adult brains. J Neurosci Res. 2007 Sep;85(12):2783-92.
Expiration:
12 months from the date of shipment when stored properly.