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Anti-GFAP Antibody, Biotinylated
Catalog #: 130-10037B-50
Rabbit anti-GFAP Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P14136 |
| Gene Id | 2670 |
| Gene Symbols | GFAP |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from Human GFAP. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000(at least detecting 12.5 µg/ml)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Neuroscience, Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Glial fibrillary acidic protein, or GFAP, is an intermediate filament (IF) protein belonging to the type III subclass of IF proteins. Like other IF proteins, GFAP is composed of an amino terminal head domain, a central rod domain and a carboxy terminal tail domain. GFAP is specifically found in astroglia, a cell type which is highly responsive to neurologic insults. Astrogliosis is found to be a result of mechanical trauma, AIDS dementia, prion infection and inflammatory demylination diseases, and is accompanied by an increase in GFAP expression. GFAP is an immunohistochemical marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat Smooth Muscle has not yet been tested.
Related Products
Rabbit Anti-Bovine GFAP(cat#DS-PB-00867) Goat Anti-Human GFAP (C-terminus)(cat#DS-PB-02261)
References
- Quinlan RA, Brenner M, Goldman JE, Messing A. GFAP and its role in Alexander disease. Exp Cell Res. 2007 Jun 10;313(10):2077-87.
- Zhu H, Dahlstr¶m A. Glial fibrillary acidic protein-expressing cells in the neurogenic regions in normal and injured adult brains. J Neurosci Res. 2007 Sep;85(12):2783-92.
Expiration:
12 months from the date of shipment when stored properly.