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Anti-GFER Antibody
Catalog #: 144-05463
GFER Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P55789 |
| Gene Id | 2671 |
| Gene Symbols | GFER |
| Protein Name / Synonyms | GFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, FAD-linked sulfhydryl oxidase ALR |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The hepatotrophic factor designated augmenter of liver regeneration (ALR) is thought to be one of the factors responsible for the extraordinary regenerative capacity of mammalian liver. It has also been called hepatic regenerative stimulation substance (HSS). The gene resides on chromosome 16 in the interval containing the locus for polycystic kidney disease (PKD1). The putative gene product is 42% similar to the scERV1 protein of yeast. The yeast scERV1 gene had been found to be essential for oxidative phosphorylation, the maintenance of mitochondrial genomes, and the cell division cycle. The human gene is both the structural and functional homolog of the yeast scERV1 gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
15kDa/23kDa
Observed MW
23kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 81-205 of human GFER (NP_005253.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GFER antibody (144-05463) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse stomach using GFER antibody (144-05463) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using GFER antibody (144-05463) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using GFER antibody (144-05463) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.