Anti-GFM1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q96RP9
Gene Id	85476
Gene Symbols	GFM1
Protein Name / Synonyms	GFM1, COXPD1, EFG, EFG1, EFGM, EGF1, GFM, hEFG1, elongation factor G, mitochondrial
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

83kDa/85kDa

Observed MW

100kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 482-751 of human GFM1 (NP_079272.4).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GFM1 antibody (144-05077) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Western blot - GFM1 Polyclonal Antibody (144-05077)

Immunohistochemistry of paraffin-embedded human liver cancer using GFM1 antibody (144-05077) at dilution of 1:100 (40x lens).
Immunohistochemistry - GFM1 Polyclonal Antibody (144-05077)

Immunohistochemistry of paraffin-embedded mouse brain using GFM1 antibody (144-05077) at dilution of 1:100 (40x lens).
Immunohistochemistry - GFM1 Polyclonal Antibody (144-05077)

Immunofluorescence analysis of U2OS cells using GFM1 antibody (144-05077) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - GFM1 Polyclonal Antibody (144-05077)

Expiration:

12 months from the date of shipment when stored properly.

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