RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-GFPT1 Antibody

Catalog #: 144-03882

GFPT1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q06210
Gene Id
2673
Gene Symbols
GFPT1
Protein Name / Synonyms
GFPT1, CMS12, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG, glutamine--fructose-6-phosphate transaminase 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Research AreaAutoimmune Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.

Dilutions

WB 1:200 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
76kDa/78kDa
Observed MW
98kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GFPT1 (NP_002047.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GFPT1 antibody (144-03882) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - GFPT1 antibody (144-03882)

Immunohistochemistry of paraffin-embedded human liver injury using GFPT1 antibody (144-03882) at dilution of 1:100 (40x lens).
Immunohistochemistry - GFPT1 antibody (144-03882)

Immunohistochemistry of paraffin-embedded rat brain using GFPT1 antibody (144-03882) at dilution of 1:100 (40x lens).
Immunohistochemistry - GFPT1 antibody (144-03882)

Expiration:
12 months from the date of shipment when stored properly.