3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-GGA2 Antibody
Catalog #: 144-01186
GGA2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9UJY4 |
| Gene Id | 23062 |
| Gene Symbols | GGA2 |
| Protein Name / Synonyms | GGA2, VEAR, ADP-ribosylation factor-binding protein GGA2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
67kDa
Observed MW
72kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GGA2 antibody (144-01186) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody (144-01186) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody (144-01186) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody (144-01186) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GGA2 Antibody (144-01186) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GGA2 Antibody (144-01186) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GGA2 antibody (144-01186). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.