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Anti-GGT1 Antibody
Catalog #: 144-01776
GGT1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P19440 |
| Gene Id | 2678 |
| Gene Symbols | GGT1 |
| Protein Name / Synonyms | GGT1, CD224, D22S672, D22S732, GGT, GGT 1, GTG, gamma-glutamyltransferase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
24kDa/39kDa/61kDa
Observed MW
60-70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 370-569 of human GGT1 (NP_001027537.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GGT1 antibody (144-01776) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using GGT1 antibody (144-01776) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using GGT1 antibody (144-01776) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GGT1 antibody (144-01776) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.