Anti-GIT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y2X7
Gene Id	28964
Gene Symbols	GIT1
Protein Name / Synonyms	GIT1, GIT ArfGAP 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW

19kDa/84kDa/85kDa

Observed MW

100kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GIT1 antibody (144-61053) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] GIT1 Polyclonal Antibody (144-61053)

Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody (144-61053) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] GIT1 Polyclonal Antibody (144-61053)

Expiration:

12 months from the date of shipment when stored properly.

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