RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-GJB3 Antibody

Catalog #: 144-02949

GJB3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O75712
Gene Id
2707
Gene Symbols
GJB3
Protein Name / Synonyms
GJB3, CX31, DFNA2, DFNA2B, EKV, EKVP1, gap junction beta-3 protein
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein.

Dilutions

WB 1:2000 - 1:50000
IHC 1:200 - 1:2000

Format

Calculated MW
30kDa
Observed MW
31kDa

Immunogen

Recombinant protein of human GJB3

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GJB3 antibody (144-02949) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - GJB3 Polyclonal Antibody (144-02949)

Expiration:
12 months from the date of shipment when stored properly.