RayBiotech

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Anti-GLB1 Antibody

Catalog #: 144-01863

GLB1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P16278
Gene Id
2720
Gene Symbols
GLB1
Protein Name / Synonyms
GLB1, EBP, ELNR1, MPS4B, beta-galactosidase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
60kDa/72kDa/76kDa
Observed MW
64kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human GLB1 (NP_001073279.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GLB1 antibody (144-01863) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - GLB1 antibody (144-01863)

Expiration:
12 months from the date of shipment when stored properly.