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Anti-GLI1 Antibody
Catalog #: 144-08387
GLI1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P08151 |
| Gene Id | 2735 |
| Gene Symbols | GLI1 |
| Protein Name / Synonyms | GLI1, GLI, zinc finger protein GLI1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
104kDa/113kDa/117kDa
Observed MW
130kDa, 160kDa
Immunogen
Recombinant protein of human GLI1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GLI1 antibody (144-08387) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.