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Anti-GLP1 Antibody
Catalog #: 130-10007
Rabbit Anti-Human GLP1 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_002045 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human GLP-1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CSSYLEGQAAKEFIAWLVKGRG |
| Recommended Applications | ELISA (recommended work dilution= 1: 4, 000(at least detecting 250ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The proglucagon gene is expressed in both pancreatic A cells and intestinal L cells. Tissue-specific post-translational processing of proglucagon by the prohormone convertase produces the different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cells produce several structurally related peptides, including glucagon-like peptide (GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation. GLP-1 has a major effect of enhancing the release of insulin in response to a glucose stimulus, and coincidentally, suppressing secretion of glucagon. GLP-1 has been shown to potently inhibit several aspects of digestive function, including gastric emptying, gastric secretion and pancreatic secretion.
Specificity
The rabbit anti-human Glucagon-like peptide-1 antibody detected specifically target derived from human recombinant protein and cellular lysates. Cross reactivity with mouse and rat GLP-1 has not yet been tested.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
References
- Tang-Christensen, M and et al., Glucagon-like peptide 1(7-36) amide's central inhibition of feeding and peripheral inhibition of drinking are abolished by neonatal monosodium glutamate treatment. Diabetes47, 530537 (1998).
- McMahon, L.R. & Wellman, P.J. PVN infusion of GLP-1-(736) amide suppresses feeding but does not induce aversion or alter locomotion in rats. Am. J. Physiol. 274, R23R29 (1998). Turton, M.D. et al. A role for glucagon-like peptide-1 in the central regulation of feeding. Nature 379, 6972 (1996).
Expiration:
12 months from the date of shipment when stored properly.