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Anti-GLP1 Antibody, Biotinylated
Catalog #: 130-10007B-50
Rabbit anti-GLP1 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NP_002045 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human GLP-1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CSSYLEGQAAKEFIAWLVKGRG |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 4, 000(at least detecting 250 µg/ml)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The proglucagon gene is expressed in both pancreatic A cells and intestinal L cells. Tissue-specific post-translational processing of proglucagon by the prohormone convertase produces the different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cells produce several structurally related peptides, including glucagon-like peptide (GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation. GLP-1 has a major effect of enhancing the release of insulin in response to a glucose stimulus, and coincidentally, suppressing secretion of glucagon. GLP-1 has been shown to potently inhibit several aspects of digestive function, including gastric emptying, gastric secretion and pancreatic secretion.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human Glucagon-like peptide-1 antibody detected specifically target derived from human recombinant protein and cellular lysates. Cross reactivity with mouse and rat GLP-1 has not yet been tested.
References
- Tang-Christensen, M and et al., Glucagon-like peptide 1(7-36) amide's central inhibition of feeding and peripheral inhibition of drinking are abolished by neonatal monosodium glutamate treatment. Diabetes47, 530537 (1998).
- McMahon, L.R. & Wellman, P.J. PVN infusion of GLP-1-(736) amide suppresses feeding but does not induce aversion or alter locomotion in rats. Am. J. Physiol. 274, R23R29 (1998). Turton, M.D. et al. A role for glucagon-like peptide-1 in the central regulation of feeding. Nature 379, 6972 (1996).
Expiration:
12 months from the date of shipment when stored properly.