GLRX Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35754 |
| Gene Id | 2745 |
| Gene Symbols | GLRX |
| Protein Name / Synonyms | GLRX, GRX, GRX1, glutaredoxin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
11kDa
Observed MW
12kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human GLRX (NP_001112362.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GLRX antibody (144-05315) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human stomach using GLRX Antibody (144-05315) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using GLRX Antibody (144-05315) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using GLRX Antibody (144-05315) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using GLRX Antibody (144-05315) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GLRX Antibody (144-05315) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GLRX antibody (144-05315). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
