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Anti-GLS Antibody
Catalog #: 144-03885
GLS Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O94925 |
| Gene Id | 2744 |
| Gene Symbols | GLS |
| Protein Name / Synonyms | GLS, AAD20, GAC, GAM, GLS1, KGA, glutaminase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
17kDa/65kDa/73kDa
Observed MW
69kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human GLS (NP_055720.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GLS antibody (144-03885) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using GLS antibody (144-03885) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using GLS antibody (144-03885) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.