3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Glucagon-like Peptide 2 Antibody
Catalog #: RB-08-0020
Rabbit Anti-Human Glucagon-like Peptide 2 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human glucagon-like peptide 2. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CTILDNLAARDFINWLI |
| Recommended Applications | ELISA (recommended work dilution= 2-4 ug), Western Blotting (recommended work dilution= 1:1000) |
| Reconstitution | Supplied as lyophilized purified antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 100 ug/ml. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The proglucagon gene is expressed in both pancreatic A cells and intestinal L cells. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produces the different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cells produce several structurally related peptides, including glucagon-like peptide (GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation. GLP-1 has a major effect of enhancing the release of insulin in response to a glucose stimulus, and coincidentally, suppressing secretion of glucagon. GLP-1 has been shown to potently inhibit several aspects of digestive function, including gastric emptying, gastric secretion and pancreatic secretion.
Specificity
The rabbit anti-human glucagon-like peptide 2 antibody detects specifically target derived from recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
References
- Larsen, P.J and et al., Central administration of glucagon-like peptide-1 activates hypothalamic neuroendocrine neurons in the rat. Endocrinology 138, 44454455 (1997).
- Tang-Christensen, M and et al., Glucagon-like peptide 1(7-36) amide's central inhibition of feeding and peripheral inhibition of drinking are abolished by neonatal monosodium glutamate treatment. Diabetes 47, 530537 (1998).
- McMahon, L.R. & Wellman, P.J. PVN infusion of GLP-1-(736) amide suppresses feeding but does not induce aversion or alter locomotion in rats. Am. J. Physiol. 274, R23R29 (1998).
- Turton, M.D. et al. A role for glucagon-like peptide-1 in the central regulation of feeding. Nature 379, 6972 (1996).
Expiration:
12 months from the date of shipment when stored properly.