Anti-GLUL Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P15104
Gene Id	2752
Gene Symbols	GLUL
Protein Name / Synonyms	GLUL, GLNS, GS, PIG43, PIG59, glutamine synthetase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

42kDa

Observed MW

40kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GLUL antibody (144-05437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] GLUL Polyclonal Antibody (144-05437)

Western blot analysis of extracts from normal (control) and GLUL knockout (KO) HeLa cells, using GLUL antibody (144-05437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - [KO Validated] GLUL Polyclonal Antibody (144-05437)

Immunohistochemistry of paraffin-embedded human stomach using GLUL Antibody (144-05437) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] GLUL Polyclonal Antibody (144-05437)

Immunohistochemistry of paraffin-embedded mouse lung using GLUL Antibody (144-05437) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] GLUL Polyclonal Antibody (144-05437)

Immunohistochemistry of paraffin-embedded mouse stomach using GLUL Antibody (144-05437) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] GLUL Polyclonal Antibody (144-05437)

Immunofluorescence analysis of MCF-7 cells using GLUL antibody (144-05437). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] GLUL Polyclonal Antibody (144-05437)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products