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Anti-GM-CSF/CSF2 Antibody
Catalog #: RB-IP-01-129
Mouse Anti-Human GM-CSF/CSF2 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_000758 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was whole expressing mouse GM-CSF. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells deriveded from the immunized mouse. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-4, 000), Western Blotting( recommended work dilution= 1-2 µg/ml) |
| Reconstitution | Supplied as purified and lyophilized antibody in sterile PBS (pH 7.5), containing 0.1% BSA. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Granulocyte-macrophage colony-stimulating factor (GM-CSF), is a protein secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts. It is a 22 kDa monomeric hematopoietic cytokine. GM-CSF-stimulated monocytes exhibit several anti-tumor behaviors. GM-CSF enhances the ability of macrophages to present antigen and initiate an immune response. It also stimulates monocytes to secrete large amounts of a soluble VEGF receptor, which binds to and inactivates VEGF.
Specificity
The antibody specifically detected protein derived from human. It also recognizes tested samples containing GM-CSF in both ELISA and WB. This antibody shows no cross-reactivity with other tested cytokines.
Storage
Always prepare single-use aliquots planning use while receiving package. A small amount of antibody can be kept at 4°C for daily use. For a long storage, recommend to keep them at -20°C after adding an equal volume of glycerol in PBS. It will not lose activity over a year. Minimize freezing and thawing when use.
References
- Armstrong, C. A., Botella, R., Galloway, T. H., Murray, N., Kramp, J. M., and Song, I. S. 1996. Antitumor effects of granulocyte-macrophage colony-stimulating factor production by melanoma cells. Cancer Research 56.2191-8.
Expiration:
12 months from the date of shipment when stored properly.