GMPS Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49915 |
| Gene Id | 8833 |
| Gene Symbols | GMPS |
| Protein Name / Synonyms | GMPS, GATD7, guanine monophosphate synthase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
65kDa/76kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 394-693 of human GMPS (NP_003866.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GMPS antibody (144-06606) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GMPS antibody (144-06606). Western blot was performed from the immunoprecipitate using GMPS antibody (144-06606) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.
