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Anti-GNAO1 Antibody
Catalog #: 144-02510
GNAO1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09471 |
| Gene Id | 2775 |
| Gene Symbols | GNAO1 |
| Protein Name / Synonyms | GNAO1, EIEE17, G-ALPHA-o, GNAO, HLA-DQB1, NEDIM, G protein subunit alpha o1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
40kDa
Observed MW
39kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human GNAO1 (NP_066268.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GNAO1 antibody (144-02510) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using GNAO1 Antibody (144-02510) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using GNAO1 Antibody (144-02510) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using GNAO1 Antibody (144-02510) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.